Nampt Antibody
| Code | Size | Price |
|---|
| PSI-36-223-100ug | 100ug | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Application Note:
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections (1:500-1:1,000)). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Background:
Nampt also known as pre-B cell colony-enhancing factor 1 (PBEF1) or visfatin is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human Nampt (visfatin/PBEF).
NCBI Gene ID #:
10135
NCBI Official Name:
nicotinamide phosphoribosyltransferase
NCBI Official Symbol:
NAMPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
5031977
Protein GI Number:
NP_005737
Research Area:
Cancer,Obesity
SPECIFICITY:
Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot.
Swissprot #:
P43490
User NOte:
Optimal dilutions for each application to be determined by the researcher.