Adiponectin Antibody [HADI 773]

ProSci
Product Code: PSI-36-001
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-001-100ug100ug£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: HADI 773
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Further Information

Additional Names:
ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa
Application Note:
ELISA: (direct and indirect: 1:5,000-1:10,000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Background:
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human adiponectin.
NCBI Gene ID #:
9370
NCBI Official Name:
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol:
ADIPOQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
295317372
Protein GI Number:
NP_001171271.1
Research Area:
Obesity
SPECIFICITY:
Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested.
Swissprot #:
Q15848
User NOte:
Optimal dilutions for each application to be determined by the researcher.