Nampt Antibody [OMNI379] (biotin)

Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase

ELISA: (direct or indirect: 1:2,000-1:10,000). Immunocytochemistry: (1:500-1:1,000). Immunohistochemistry: (paraffin sections, 1:100-1:500). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

1 mg/ml

Biotin

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Recombinant human Nampt (visfatin/PBEF).

10135

nicotinamide phosphoribosyltransferase

NAMPT

Homo sapiens

Liquid

5031977

NP_005737

Cancer,Obesity

Recognizes human, mouse and rat Nampt.

P43490

Optimal dilutions for each application to be determined by the researcher.