PAG/Cbp Antibody
| Code | Size | Price |
|---|
| PSI-36-345-100ug | 100ug | £838.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Immunocytochemistry (ICC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
Phosphoprotein Associated with Glycosphingolipid-enriched Microdomains 1; Csk-binding Protein
Application Note:
Immunocytochemistry: (1:50). Immunoprecipitation: (5ug/sample) . Western Blot: (1:1,000) . Optimal conditions must be determined individually for each application.
Background:
PAG [Cbp] is a ubiquitously expressed transmembrane adapter protein. Depending on its phosphorylation status, it appears as diffuse band(s) of 80-90kDa by SDS-PAGE, with phosphorylation leading to a shift towards a higher apparent molecular mass. Tyrosine phosphorylated PAG recruits Csk to the membrane and negatively regulates Src family kinases via Csk-mediated phosphorylation. Upon T cell receptor engagement, PAG becomes dephosphorylated, thereby displacing Csk from the membrane and enabling the activation of the Src kinases Fyn and Lck. PAG negatively regulates TCR (T cell antigen receptor)-mediated signaling in T cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. It promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. It inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins and may be involved in cell adhesion signaling.
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Synthetic peptide corresponding to aa 412-424 of the N-terminal rat PAG including an additional N-terminal Cys residue.
NCBI Gene ID #:
64019
NCBI Official Name:
phosphoprotein membrane anchor with glycosphingolipid microdomains 1
NCBI Official Symbol:
Pag1
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
Protein Accession #:
81868799
Protein GI Number:
Q9JM80
Purification:
Antigen affinity purified.
Research Area:
Immunology,Innate Immunity
SPECIFICITY:
Recognizes mouse, rat and human PAG/Cbp.
Swissprot #:
Q9JM80
User NOte:
Optimal dilutions for each application to be determined by the researcher.