BHLHA15 Antibody

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ProSci
Product Code: PSI-8541
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8541-0.02mg0.02mg£150.00
Quantity:
PSI-8541-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
BHLHA15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.
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Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.

Documents

Further Information

Additional Names:
BHLHA15 Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E
Application Note:
BHLHA15 antibody can be used for Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
BHLHA15 Antibody: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
Background References:
  • Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13.
  • Pin CL, Rukstalis JM, Johnson C, et al. The bHLH transcription factor MIST1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J. Cell Biol. 2001; 155:519-30.
  • Lemercier C, To RQ, Carrasco RA et al. The basic helix-loop-helix transcription factor Mist1 functions as a transcriptional repressor of myoD. EMBO J. 1998; 17:1412-22.
  • Zhao Y, Johansson C, Tran T, et al. Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required for maintenance of the differentiated state. Mol. Endocrinol. 2006; 20:2187-98.
Buffer:
BHLHA15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
BHLHA15 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BHLHA15.
NCBI Gene ID #:
168620
NCBI Official Name:
basic helix-loop-helix family member a15
NCBI Official Symbol:
BHLHA15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 21 kDa

Observed: 22 kDa
Protein Accession #:
NP_803238
Protein GI Number:
29126247
Purification:
BHLHA15 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction,Stem Cell
Swissprot #:
Q7RTS1
User NOte:
Optimal dilutions for each application to be determined by the researcher.