Caspase-12 Antibody [12G6]
| Code | Size | Price |
|---|
| PSI-36-184-100ug | 100ug | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 12G6
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
CASP-12
Application Note:
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunoprecipitation: (1:500-1:5,000). Western Blot: (1:500-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Background:
Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12.
NCBI Gene ID #:
12364
NCBI Official Name:
caspase 12
NCBI Official Symbol:
Casp12
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
2094806
Protein GI Number:
CAA73532
Purification:
>95% (SDS-PAGE)
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11.
Swissprot #:
O08736
User NOte:
Optimal dilutions for each application to be determined by the researcher.