Caspase-8 Antibody [1G12]
| Code | Size | Price |
|---|
| PSI-36-180-100ug | 100ug | £597.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 1G12
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Immunocytochemistry (ICC)
- Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease
Application Note:
ELISA: (recombinant mouse caspase-8) . Flow Cytometry: (overexpressed in cell lines) . Immunocytochemistry: (see literature reference 2) . Western Blot: (excellent) . Optimal conditions must be determined individually for each application.
Background:
Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p20, respectively, which leads to the generation of the active caspase-8 heterotetramer (p20/p10)2.
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant mouse caspase-8 (p20 subunit).
NCBI Gene ID #:
12370
NCBI Official Name:
caspase 8
NCBI Official Symbol:
Casp8
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
25090576
Protein GI Number:
O89110
Purification:
>95% (SDS-PAGE)
Research Area:
Apoptosis,Cancer
SPECIFICITY:
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
Swissprot #:
O89110
User NOte:
Optimal dilutions for each application to be determined by the researcher.