FTO Antibody [FT62-6]

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ProSci
Product Code: PSI-36-084
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-084-100ug100ug£577.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: FT62-6
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
Fat Mass and Obesity-associated Protein; FATSO
Application Note:
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections (1:100-1:500)). Immunoprecipitation: (5ug/ml per sample in a final reaction volume of 500-1000ul). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Background:
FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse ?fused toes? mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human FTO.
NCBI Gene ID #:
26383
NCBI Official Name:
fat mass and obesity associated
NCBI Official Symbol:
Fto
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
81873739
Protein GI Number:
Q8BGW1
Research Area:
Obesity
SPECIFICITY:
Recognizes mouse FTO. Weakly cross-reacts with human FTO. Does not cross-react with rat FTO.
Swissprot #:
Q8BGW1
User NOte:
Optimal dilutions for each application to be determined by the researcher.