Caspase-11 Antibody [4E11]

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ProSci
Product Code: PSI-36-183
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-36-183-100ug100ug£638.00
Quantity:
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Overview

Host Type: Rat
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 4E11
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Immunocytochemistry (ICC)
  • Western Blot (WB)
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
Caspase-11 (mouse); Protease ICH-3 (mouse)
Application Note:
ELISA: (recombinant protein). Flow Cytometry: (overexpressed in cell lines). Immunoprecipitation: (1-5ug/ml). Western Blot: (1-2ug/ml) . Optimal conditions must be determined individually for each application.
Background:
Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18), but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.
Buffer:
Liquid. In PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant mouse caspase-11 (p20 fragment).
NCBI Gene ID #:
12363
NCBI Official Name:
caspase 4, apoptosis-related cysteine peptidase
NCBI Official Symbol:
Casp4
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
341940314
Protein GI Number:
P70343
Purification:
>95% (SDS-PAGE)
Research Area:
Apoptosis,Cancer,Innate Immunity
SPECIFICITY:
Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot.
Swissprot #:
P70343
User NOte:
Optimal dilutions for each application to be determined by the researcher.