Leptin Antibody
| Code | Size | Price |
|---|
| PSI-36-200-100ug | 100ug | £541.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
OB Gene Product; Obese Factor
Application Note:
ELISA: (direct and indirect: 1:5,000-1:10,000). Western Blot: (1:5,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Background:
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant rat leptin.
NCBI Gene ID #:
25608
NCBI Official Name:
leptin
NCBI Official Symbol:
Lep
NCBI Organism:
Rattus norvegicus
Physical State:
Liquid
Protein Accession #:
1709436
Protein GI Number:
P50596
Research Area:
Obesity
SPECIFICITY:
Recognizes rat leptin. Weakly cross-reacts with human and mouse leptin. Detects a band of ~16kDa by Western blot.
Swissprot #:
P50596
User NOte:
Optimal dilutions for each application to be determined by the researcher.