NAD Kinase M. tuberculosis Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-365-50ug | 50ug | £673.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Dry ice
Storage:
Stable for at least 6 months after receipt when stored at -80˚C.
Documents
Further Information
Additional Names:
Probable Inorganic Polyphosphate/ATP-NAD kinase, Poly(P)/ATP NAD Kinase 1, ppnK1, EC 2.7.1.23
Application Note:
This recombinant proteins is for research use only.
Background:
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
Biological Activity:
~1.2 U/mg protein. (Substrate: NAD (+ ATP))
~3.8 U/mg protein. (Substrate: NAD (+ poly(P))
One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min.
Buffer:
In 10mM TRIS at pH 8.0, containing 1mM MgCl2, 0.5mM EDTA, 1mM DTT and 1mM PMSF.
Concentration:
0.5mg/ml (Lot specific)
Fusion Tag:
His Tag
Physical State:
Liquid
Protein Accession #:
P0A5S6
Purity:
>98% (SDS-PAGE)
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q07011