NAD Nucleotidase H. influenzae Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-368-50ug | 50ug | £782.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Dry ice
Storage:
Stable for at least 6 months after receipt when stored at -80˚C.
Documents
Further Information
Additional Names:
NadN, NAD Pyrophosphatase/5'-nucleotidase
Application Note:
This recombinant proteins is for research use only.
Background:
NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside). Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi
Biological Activity:
With AMP: 856.4 nmol of Ade/min/mg of protein
With NMN: 110,3 nmol of NR/min/mg of protein
With NAD: 5.4 nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein
Buffer:
In 5mM KH2PO4, pH 6.8 containing 100mM TRIS-HCl (pH 8.5) and 10mM MgCl2.
Concentration:
0.5mg/ml (Lot specific)
Fusion Tag:
Tag Free
NCBI Gene ID #:
3429631
NCBI Official Name:
5'-nucleotidase?
NCBI Official Symbol:
nadN
NCBI Organism:
Haemophilus influenzae 86-028NP?
Physical State:
Liquid
Protein Accession #:
Q4QNY4
Protein GI Number:
81336775
Purity:
>98% (SDS-PAGE)
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P16410