CD152 [CTLA-4] non-lytic Recombinant Protein

Product Code: PSI-90-413

Supplier: ProSci

Code	Size	Price

PSI-90-413-100ug

100ug

£373.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO

Target Species: Human

Storage:

Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.

Documents

Datasheet

Further Information

Additional Names:

CTLA-4, Cytotoxic T-lymphocyte Protein 4

Application Note:

This recombinant proteins is for research use only.

Background:

CD152 (CTLA-4) and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD152 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CD152 and CD28 are composed of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. CD152 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. CD152 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CD152 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CD152 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CD152 and CD28 can bind to the same ligands, CD152 binds to B71 and B72 with 20-100-fold higher affinity than CD28.

Biological Activity:

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells.

Buffer:

Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 μg/ml in sterile PBS.

Fusion Tag:

Fc Tag

NCBI Gene ID #:

1493

NCBI Official Name:

cytotoxic T-lymphocyte associated protein 4

NCBI Official Symbol:

CTLA4

NCBI Organism:

Homo sapiens

Physical State:

Lyophilized

Protein Accession #:

NP_005205

Protein GI Number:

21361212

Purity:

>98% (SDS-PAGE).

Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).

Recombinant Protein Sequence:

Thr32-Gly402

Source:

CHO cells

Swissprot #:

P01583