B7-H4 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-369-100ug | 100ug | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Stable for at least 1 year after receipt when stored at -20˚C. Working aliquots are stable for up to 3 months when stored at -20˚C.
Documents
Further Information
Additional Names:
V-set Domain-containing T Cell Activation Inhibitor 1, VTCN1, B7h.5, Immune Costimulatory Protein B7-H4, T Cell Costimulatory Molecule B7x, Protein B7S1
Application Note:
This recombinant proteins is for research use only.
Background:
B7H4 is a B7 family member that negatively regulates T cell immunity by inhibiting of T cell proliferation, cytokine production, and cell cycle progression. In vitro, B7H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.
Biological Activity:
Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well place coated with 500 ng/ml anti-CD3 and 10ug/ml human B7H4/Fc. The presence of human B7H4/Fc at 10ug/ml inhibited anti CD3 response by 30-50%. Optimal dilutions should be determined by each laboratory for each application.
Buffer:
Lyophilized from 0.2um-filtered solution in PBS. Reconstitute with 100 μl sterile water. Add 1X PBS to the desired protein concentration.
Fusion Tag:
Fc Tag
NCBI Gene ID #:
79679
NCBI Official Name:
V-set domain containing T cell activation inhibitor 1
NCBI Official Symbol:
VTCN1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
NP_078902
Protein GI Number:
99028881
Purity:
>98% (SDS-PAGE).
Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
Endotoxin level is less than 0.06EU/ μg protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
CHO cells
Swissprot #:
P16410