LIF Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-277-10ug | 10ug | £404.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor
Application Note:
This recombinant proteins is for research use only.
Background:
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R alpha that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Biological Activity:
Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF.
Buffer:
Contains PBS. Reconstitute with 100 μl sterile water.
Concentration:
0.1mg/ml after reconstitution
Fusion Tag:
GST tag
NCBI Gene ID #:
3976
NCBI Official Name:
leukemia inhibitory factor
NCBI Official Symbol:
LIF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P15018
Protein GI Number:
4504991
Purity:
>95% (SDS-PAGE).
Endotoxin level is less than 0.05EU/ μg purified protein (LAL test; Lonza).
Endotoxin level is less than 0.05EU/ μg purified protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P13597