Flagellin NLRC4 Mutant Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-306-10ug | 10ug | £469.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
FliC (NLRC4 Mutant)
Application Note:
This recombinant proteins is for research use only.
Background:
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (NLRC4 Mutant)(rec. ) is only detected by TLR5 not by NLRC4.
Biological Activity:
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at a concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages. It can be used as negative control for inflammasome signaling.
Buffer:
Contains PBS. Reconstitute with 100 μl sterile water.
Concentration:
0.1mg/ml after reconstitution.
Fusion Tag:
His Tag
NCBI Gene ID #:
1253480
NCBI Official Name:
flagellin
NCBI Official Symbol:
fliC
NCBI Organism:
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Physical State:
Lyophilized
Protein Accession #:
NP_460912
Protein GI Number:
16765297
Purity:
>95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q8NAU1