TRAIL, Soluble Recombinant Protein

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ProSci
Product Code: PSI-90-217
Product Group: Recombinant Proteins
Supplier: ProSci
CodeSizePrice
PSI-90-217-10ug10ug£469.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Storage:
Stable for at least 6 months after receipt when stored at -20˚C.

Documents

Further Information

Additional Names:
Apo-2L, TNFSF10, CD253
Application Note:
This recombinant proteins is for research use only.
Background:
TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to fi ve distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2;TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducingthe formation of the so-called multiprotein death-inducing signaling complex (DISC).
Biological Activity:
Induces apoptosis of human Jurkat T cells in a concentration range of 5-50ng/ml in the presence of 1ug/ml TNF Ligands Enhancer (Prod. No. AG-35B-0001). In the absence of TNF Ligands Enhancer, TRAIL, Soluble (human) (recombinant) is poorly active.
Buffer:
Contains PBS. Reconstitute with 100 μl sterile water.
Concentration:
0.1mg/ml after reconstitution.
Fusion Tag:
DDDDK Tag
NCBI Gene ID #:
8743
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 10
NCBI Official Symbol:
TNFSF10
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P50591
Protein GI Number:
4507593
Purity:
>95% (SDS-PAGE).

Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P08758