TNF-alpha, Soluble Recombinant Protein
| Code | Size | Price |
|---|
| PSI-90-222-50ug | 50ug | £469.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Storage:
Stable for at least 1 year after receipt when stored at -20˚C.
Documents
Further Information
Additional Names:
Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin
Application Note:
This recombinant proteins is for research use only.
Background:
Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (MultimericTNF-alpha (Prod. No. AG-40B-0019). Since the MultimericTNF-alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha can be used.
Biological Activity:
Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Buffer:
Contains PBS. Reconstitute with 50 μl sterile water.PBS containing at least 0.1% BSA should be used for further dilutions.
Concentration:
10 ug size: 0.1 mg/ml after reconstitution.50 ug size: 1 mg/ml after reconstitution.
Fusion Tag:
DDDDK Tag
NCBI Gene ID #:
7124
NCBI Official Name:
tumor necrosis factor
NCBI Official Symbol:
TNF
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01375
Protein GI Number:
135934
Purity:
>95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
O14788