IL-1 beta Recombinant Protein
| Code | Size | Price |
|---|
| PSI-91-032-0.05mg | 0.05mg | £838.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Storage:
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
Documents
Further Information
Additional Names:
Interleukin-1 Beta, IL-1 Beta, Il1b, IL-1F2, IL-1b
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Interleukin-1 (IL-1) designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. The specific protease responsible for the processing of IL-1 beta , designated interleukin 1 beta -converting enzyme (ICE), has been described. Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines.
Biological Activity:
ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells.
Specific Activity of 1.0 x 108 IU/mg.
Specific Activity of 1.0 x 108 IU/mg.
Buffer:
Lyophilized from a 0.2 um filtered solution of 50mM TrisHCl, 50mM NaCl, pH 8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
Tag Free
NCBI Gene ID #:
16176
NCBI Official Name:
interleukin 1 beta
NCBI Official Symbol:
Il1b
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
17.4 kD
Protein Accession #:
P10749
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P10749