Interferon-stimulated Gene 15 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-92-169-0.05mg | 0.05mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Documents
Further Information
Additional Names:
Ubiquitin-Like Protein ISG15, Interferon-Induced 15 kDa Protein, Interferon-Induced 17 kDa Protein, IP17, Ubiquitin Cross-Reactive Protein, hUCRP, ISG15, G1P2, UCRP
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.
Buffer:
Supplied as a 0.2 um filtered solution of 50mM HEPES, 100mM NaCl, pH 8.0. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Fusion Tag:
C-6 His tag
NCBI Gene ID #:
9636
NCBI Official Name:
ISG15 ubiquitin-like modifier
NCBI Official Symbol:
ISG15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
18.2 kD
Protein Accession #:
P05161
Protein GI Number:
189065303
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P05161