Alkaline Phosphatase Recombinant Protein
| Code | Size | Price |
|---|
| PSI-91-503-0.05mg | 0.05mg | £883.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Documents
Further Information
Additional Names:
Alkaline Phosphatase, Tissue-Nonspecific Isozyme, AP-TNAP, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme, ALPL
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP(PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia,characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.
Buffer:
Supplied as a 0.2 um filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Fusion Tag:
C-6 His tag
NCBI Gene ID #:
249
NCBI Official Name:
alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol:
ALPL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
54.5 kD
Protein Accession #:
P05186
Protein GI Number:
578798860
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
Human Cells
Swissprot #:
P05186