Cyclin-D2 Recombinant Protein
| Code | Size | Price |
|---|
| PSI-92-305-0.05mg | 0.05mg | £673.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Storage:
Store at -20˚C, stable for 6 months after receipt.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Documents
Further Information
Additional Names:
G1/S-specific cyclin-D2, CCND2
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
CCND2, also known as G1/S-specific cyclin-D2, is a member of the highly conserved cyclin family. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND2 is involved in a number of fundamental biological processes such as phosphorylating and inhibiting members of the retinoblastoma (RB) protein family including RB1 and regulating the cell-cycle during G1/S transition. It is also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Buffer:
Supplied as a 0.2 um filtered solution of 20mM Tris,150mM NaCl,5mM DTT,pH7.5. It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Fusion Tag:
N-6 His tag
NCBI Gene ID #:
894
NCBI Official Name:
cyclin D2
NCBI Official Symbol:
CCND2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Predicted Molecular Weight:
35.36 kD
Protein Accession #:
P30279
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
P30279