Sonic Hedgehog Recombinant Protein
| Code | Size | Price |
|---|
| PSI-92-281-0.05mg | 0.05mg | £657.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Mouse
Storage:
Lyophilized protein should be stored at -20˚C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
Reconstituted protein solution can be stored at 4-7˚C for 2-7 days.
Aliquots of reconstituted samples are stable at -20˚C for 3 months.
Documents
Further Information
Additional Names:
Sonic Hedgehog Protein, SHH, HHG-1, SHH(Cys25Ile-Ile)
Application Note:
This recombinant protein can be used for biological assays. For research use only.
Background:
Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa N?terminal fragment (Shh?N), and a catalytic?processing,glycosylated, 34 kDa, 239 aa C?terminal fragment. The 20 kDa Shh?N fragment is the core of the active hedgehog molecule. Mouse Shh?N is 99%, 98%, and 100% aa identical to human, rat and gerbil Shh?N, respectively.
Buffer:
Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O.
Fusion Tag:
Tag Free
NCBI Gene ID #:
20423
NCBI Official Name:
sonic hedgehog
NCBI Official Symbol:
Shh
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Predicted Molecular Weight:
19.8 kD
Protein Accession #:
Q62226
Purity:
Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
E. coli
Swissprot #:
Q62226