HLA-B monoclonal antibody, clone SPM420

Product Code: MAB13074

Code	Size	Price

MAB13074-100ug

100 ug

£488.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG3, kappa

Antibody Clonality: Monoclonal

Antibody Clone: SPM420

Regulatory Status: RUO

Target Species: Human

Applications:

Flow Cytometry
Immunofluorescence (IF)

Storage:

Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.

Documents

Supplier External Link

Further Information

Antigen Target Species:

Human

Form:

Liquid

Gene Alias:

Gene Description:

major histocompatibility complex, class I, B

Gene ID:

3106

Gene Name:

HLA-B

Immunogen:

Normal human peripheral blood lymphocytes.

Product Description:

Mouse monoclonal antibody raised against native human HLA-B.

Protein Accession:

P01889

Recommend Dilutions:

Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.

Specificity:

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

Storage Buffer:

In 10 mM PBS.