CD45RA monoclonal antibody, clone PTPRC/818

Human

Liquid

B220|CD45|CD45R|GP180|LCA|LY5|T200

protein tyrosine phosphatase, receptor type, C

5788

PTPRC

Recombinant protein corresponding to full length human CD45RA.

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Mouse monoclonal antibody raised against full length recombinant human CD45RA.

Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.

Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

In 10 mM PBS (0.05% BSA, 0.05% sodium azide).