HLA-DQ monoclonal antibody, clone SPM422
| Code | Size | Price |
|---|
| MAB13078-100ug | 100 ug | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM422
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
Documents
Further Information
Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
CD|CELIAC1|DQ-A1|FLJ27088|FLJ27328|GSE|HLA-DQA|MGC149527
Gene Description:
major histocompatibility complex, class II, DQ alpha 1
Gene ID:
3117|3118|3119
Gene Name:
HLA-DQA1
Immunogen:
T4-positive CTL clone HG-38.
Product Description:
Mouse monoclonal antibody raised against native human HLA-DQ.
Protein Accession:
P01908;P01909;P01920
Recommend Dilutions:
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
Specificity:
Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. This monoclonal antibody strongly blocks cytotoxicity activity of T4+ cytotoxic T cell clones.
Storage Buffer:
In 10 mM PBS.