CD45RA monoclonal antibody, clone SPM504
| Code | Size | Price |
|---|
| MAB13150-100ug | 100 ug | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM504
Regulatory Status: RUO
Target Species: Human
Applications:
- Flow Cytometry
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
Documents
Further Information
Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
B220|CD45|CD45R|GP180|LCA|LY5|T200
Gene Description:
protein tyrosine phosphatase, receptor type, C
Gene ID:
5788
Gene Name:
PTPRC
Immunogen:
Stimulated human leukocytes.
Product Description:
Mouse monoclonal antibody raised against native human CD45RA.
Recommend Dilutions:
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
Specificity:
Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells, B cells, and leukemic B cell lines. T cells expressing CD45RA are naive or virgin T cells. T cells expressing CD45RO are memory T cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T cells. It can especially be used to differentiate T cell lymphomas (CD45RO+ve) from B cell lymphomas (CD45RA+ve).
Storage Buffer:
In 10 mM PBS.