HLA-DRB monoclonal antibody, clone SPM289

Human

Liquid

DRB1|FLJ76359|HLA-DR1B|HLA-DRB|HLA-DRB1*|SS1

major histocompatibility complex, class II, DR beta 1

3123

HLA-DRB1

Activated human peripheral blood mononuclear cells.

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Mouse monoclonal antibody raised against native human HLA-DRB.

P01911

Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be d

This monoclonal antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the HLA-DRB monoclonal antibody, clone SPM289, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4⁺ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

In 10 mM PBS (0.05% BSA, 0.05% sodium azide).