EPHA2 recombinant monoclonal antibody clone 1C1

Abnova Corporation
Product Code: RAB00035
Product Group: Primary Antibodies
CodeSizePrice
RAB00035-200ug200 ug£709.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Isotype: IgG1 kappa
Antibody Clonality: Recombinant Antibody
Antibody Clone: 1C1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4°C for up to 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Further Information

Antigen Target Species:
Human
Form:
Liquid
Gene Alias:
ECK
Gene Description:
EPH receptor A2
Gene ID:
1969
Gene Name:
EPHA2
Immunogen:
Original antibody is raised against recombinant protein corresponding to residues within 183-510 of human EPHA2.
Preparation Method:
1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
Product Description:
Humanized recombinant monoclonal antibody raised against human EPHA2.
Protein Accession:
P29317
Recommend Dilutions:
ELISA (0.1 ug/mL)
Flow Cytometry (1 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.
Specificity:
1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.
Storage Buffer:
In PBS, pH 7.2 (0.02% Proclin 300).