ETV4

Contact us
ProSci
Product Code: PSI-8535
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8535-0.02mg0.02mg£150.00
Quantity:
PSI-8535-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
ETV4 antibody can be stored at 4C for three months and -20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 1
Western blot analysis of ETV4 in (A) human brain tissue lysate and (B) A431 cell lysate with ETV4 antibody at 1 μg/ml.
❮❯

Western blot analysis of ETV4 in (A) human brain tissue lysate and (B) A431 cell lysate with ETV4 antibody at 1 μg/ml.

Documents

Further Information

Additional Names:
ETV4 Antibody: ETS variant 4, E1AF, PEA3, E1A-F, PEAS3
Application Note:
ETV4 antibody can be used for Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
ETV4 Antibody: ETV4, along with the related proteins ETV1 and ETV5, form the PEA3 subfamily of the E26 transformation-specific (ETS) transcription factor family (1). ETV4 is thought to play roles in embryonic kidney development and fertility (2,3), and is also thought to be an oncoprotein in multiple cancers (4).
Background References:
  • Xin JH, Cowie A, LAchance P, et al. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells. Genes Dev. 1992; 6:481-96.
  • Lu BC, Cebrian C, Chi X, et al. Etv4 and Etv5 are required downstream of GDNF and Ret for kidney branching morphogenesis. Nat. Genet. 2009; 41:1295-302.
  • Laing MA, Coonrod S, Hinton BT, et al. Male sexual dysfunction in mice bearing targeted mutant alleles of the PEA3 ets gene. Mol. Cell. Biol. 2000; 20:9337-45.
  • Oh S, Shin S, and Janknecht R. ETV1, 4 and 5: An oncogenic subfamily of ETS transcription factors. Biochim. Biophys. Acta 2012: 1826:1-12.
Buffer:
ETV4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish: (72%)
Immunogen:
ETV4 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human ETV4.
NCBI Gene ID #:
2118
NCBI Official Name:
ETS translocation varinat 4
NCBI Official Symbol:
ETV4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 53 kDa

Observed: 53 kDa
Protein Accession #:
NP_001977
Protein GI Number:
24307883
Purification:
ETV4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of ETV4 are known to exist. This antibody will only detect the two longest isoforms.
Swissprot #:
P43268
User NOte:
Optimal dilutions for each application to be determined by the researcher.