Anti-Human CD45RO Monoclonal Antibody, Unconjugated Clone UCHL1

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Diaclone
Product Code: 854.333.020
Product Group: Primary Antibodies
Supplier: Diaclone
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854.333.020100ug/100ul£371.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: UCHL1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
Storage:
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

Documents

Further Information

Antigen:
CD45RO
Conjugate:
Purified
Format:
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Immunisation:
Human IL-2 dependent T cells
Reactivity:
Human
Research Area:
Non Lineage Markers,CD Markers
Specificity:
The antibody UCHL1 recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.
Working Dilution:
Use 10 µl of the suggested working dilution to label 106 cells in 100 µl.

References

  1. Akbar A.N. et al., J Immunol., 1988;140(7):2171-8. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.. https://www.ncbi.nlm.nih.gov/pubmed/2965180
  2. Beverley P.C. et al., Immunol Suppl.,1988;1:3-5. Phenotypic diversity of the CD45 antigen and its relationship to function.. https://www.ncbi.nlm.nih.gov/pubmed/2968306
  3. Beverley P.C., Immunol Lett., 1987;14(4):263-7. Human T cell subsets.. https://www.ncbi.nlm.nih.gov/pubmed/2953677
  4. Bijian K. et al., Mol Immunol., 2007;44(15):3682-90. Collagen-mediated survival signaling is modulated by CD45 in Jurkat T cells.. https://www.ncbi.nlm.nih.gov/pubmed/17524482
  5. Cosenza-Nashat M.A. et al., Brain Pathol. ,2006;16(4):256-65. CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis.. https://www.ncbi.nlm.nih.gov/pubmed/17107594
  6. Dawes R. et al., J Immunol., 2006;176(6):3417-25. Combinations of CD45 isoforms are crucial for immune function and disease.. https://www.ncbi.nlm.nih.gov/pubmed/16517710
  7. Desharnais P. et al., Apoptosis.,2007 Dec 19. Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.
  8. Leucocyte Typing IV. Knapp W et al. (Eds.), Oxford University Press (1989).. .
  9. Leucocyte Typing V. Schlossman S. et al. (Eds.), Oxford University Press (1995).. .
  10. Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987). .
  11. Li F.J. et al., Blood, 2005;105(8):3295-302. A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma.. https://www.ncbi.nlm.nih.gov/pubmed/15626731
  12. Merkenschlager M. et al., Eur J Immunol.,1988;18(11):1653-61. Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.. https://www.ncbi.nlm.nih.gov/pubmed/2974420
  13. Norton A.J. et al., J Clin Pathol., 1986;39(4):399-405. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.. https://www.ncbi.nlm.nih.gov/pubmed/2422219
  14. Smith S.H. et al., Immunology, 1986;58(1):63-70. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.. https://www.ncbi.nlm.nih.gov/pubmed/2423439
  15. Terry L.A. et al., Immunology, 1988;64(2):331-6. The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.. https://www.ncbi.nlm.nih.gov/pubmed/2455685