FURA 2-AM
| Code | Size | Price |
|---|
| CDX-F0014-M001 | 1 mg | £157.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
Short Term: -20°C . Long Term: -20°C
Documents
Further Information
Alternate Names/Synonyms:
FURA-2 acetoxymethyl ester; FURA-2 pentakis(acetoxymethyl) ester
Appearance:
Yellow solid.
CAS:
108964-32-5
EClass:
32160000
Form (Short):
solid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
InChiKey:
VPSRLGDRGCKUTK-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 108964-32-5. Formula: C44H47N3O24. MW: 1001.87. Synthetic. The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (lambdaex = 362nm, lambdaem = 512nm) differ from those during high calcium conditions (lambdaex = 335nm, lambdaem = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: lambdaex 355nm; lambdaem 495nm in 0.1 M Tris pH 8.0.
MDL:
MFCD00036976
Molecular Formula:
C44H47N3O24
Molecular Weight:
1001.87
Package Type:
Vial
Product Description:
The fluorescent probe FURA 2/AM is a membrane-permeable derivative of the calcium chelator FURA 2. Inside cells, FURA 2/AM is rapidly hydrolyzed by cytoplasmic esterases to FURA 2, which remains within the cell. Non-invasive, intracellular calcium indicator that is UV light-excitable. Fluorescent Ca2+ indicator, selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA 2/AM is used to determine calcium concentrations in many cellular systems, including: cardiac myocytes, vascular smooth muscle cells and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (lambdaex = 362nm, lambdaem = 512nm) differ from those during high calcium conditions (lambdaex = 335nm, lambdaem = 505nm). FURA 2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Spectral Data: lambdaex 355nm; lambdaem 495nm in 0.1 M Tris pH 8.0.
Purity:
>95% (HPLC)
SMILES:
O=C(OCOC(C)=O)C1=CN=C(O1)C2=CC3=C(O2)C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C3
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) G. Grynkiewicz, et al.; J. Biol. Chem. 260, 3440 (1985) | (2) I. Maruyama, et al.; FEBS Lett. 220, 89 (1987) | (3) M.E. Janconi, et al.; J. Cell Biol. 110, 1555 (1990) | (4) Y. Schwartz, et al.; Endocrinology 131, 772 (1992) | (5) G.H. Rao, et al.; Biochem. Med. Metab. Biol. 47, 226 (1992) | (6) M.J.Davis, et al.; Am. J. Physiol. 263, H1292 (1992) | (7) M.J. Davis, et al.; Am. J. Physiol. 263, H1292 (1992) | (8) M.L. Field, et al.; Cell Calcium. 16, 87 (1994) | (9) R.W. Sabnis; Handbook of biological dyes and stains (2010)