Zincon monosodium salt

2-[5-(2-Hydroxy-5-sulfophenyl)-3-phenyl-1-formazyl]benzoic acid monosodium salt; 2-Carboxy-2'-hydroxy-5'-sulfoformazyl-benzene monosodium salt

Dark violet powder.

62625-22-3

32160000

liquid

GHS07

Protect from light and moisture.

H315, H319, H335

InChI=1S/C20H16N4O6S.Na/c25-18-11-10-14(31(28,29)30)12-17(18)22-24-19(13-6-2-1-3-7-13)23-21-16-9-5-4-8-15(16)20(26)27;/h1-12,21,25H,(H,26,27)(H,28,29,30);/q;+1/p-1/b23-19+,24-22+;

DVHNWVSFUULGKM-SWJPHGMYSA-M

Chemical. CAS: 62625-22-3. Formula: C20H15N4NaO6S. MW: 462.41. Synthetic. A colorimetric reagent for Zn, Cu and Hg detection and a metal indicator in EDTA titration. The aqueous solution of Zincon is yellow or orange-yellow in weak alkaline conditions, and turns blue in the presence of Cu (maximum wavelength: 600nm, molar absorptivity: 1.9x104) and Zn (pH 8.5-9.5, maximum wavelength: 620nm, molar absorptivity: 23,000). Used for the spectrophotometric determination of zinc, simultaneous determination of zinc and copper and for automated flow injection spectrometric determination of zinc. Binds nonspecifically proteins at acidic pH and was therefore used to stain proteins fast and background-free in sodium dodecyl sulfate polyacrylamide gel (SDS-Page). Sligthly less sensitivity than Coomassie Brilliant Blue®CBB but stains and destains more rapidly. Additionally, zincon staining is reversible, suggesting it might be applicable to 2-D PAGE and downstream analyses by mass spectrometry. Shown to inhibit insulin binding to its receptor and to attenuate the activity of ATPase and other enzymes, presumably because of the chelating properties of the dye.

MFCD00064385

C20H15N4NaO6S

462.41

Vial

P305, P351, P338

A colorimetric reagent for Zn, Cu and Hg detection and a metal indicator in EDTA titration. The aqueous solution of Zincon is yellow or orange-yellow in weak alkaline conditions, and turns blue in the presence of Cu (maximum wavelength: 600nm, molar absorptivity: 1.9x104) and Zn (pH 8.5-9.5, maximum wavelength: 620nm, molar absorptivity: 23,000). Used for the spectrophotometric determination of zinc, simultaneous determination of zinc and copper and for automated flow injection spectrometric determination of zinc. Binds nonspecifically proteins at acidic pH and was therefore used to stain proteins fast and background-free in sodium dodecyl sulfate polyacrylamide gel (SDS-Page). Sligthly less sensitivity than Coomassie Brilliant Blue®CBB but stains and destains more rapidly. Additionally, zincon staining is reversible, suggesting it might be applicable to 2-D PAGE and downstream analyses by mass spectrometry. Shown to inhibit insulin binding to its receptor and to attenuate the activity of ATPase and other enzymes, presumably because of the chelating properties of the dye.

~85%

Warning

O=S(C1=CC=C(O)C(/N=N/C(C2=CC=CC=C2)=N/NC3=CC=CC=C3C(O)=O)=C1)([O-])=O.[Na+]

Slightly soluble in water (20°C), alcohol or aqueous alkali. Insoluble in most organic solvents.

Synthetic.

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at +20°C.