CPT1A Antibody

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ProSci
Product Code: PSI-61-413
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-413-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-CPT1A Pab in Y79 cell line lysate (35ug/lane).
2 / 2
Left image is paraformaldehyde-fixed and paraffin-embedded cow lactating with CPT1A Pab , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining, right image is contrast, did not add the antibody. This data was kindly offered by Hideaki Hayashi, University of Bern, Switzerland.
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Western blot analysis of anti-CPT1A Pab in Y79 cell line lysate (35ug/lane).
Left image is paraformaldehyde-fixed and paraffin-embedded cow lactating with CPT1A Pab , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining, right image is contrast, did not add the antibody. This data was kindly offered by Hideaki Hayashi, University of Bern, Switzerland.

Documents

Further Information

Additional Names:
Carnitine O-palmitoyltransferase 1, liver isoform, CPT1-L, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPTI-L, Carnitine palmitoyltransferase 1A, CPT1A, CPT1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.
Background References:
  • Rasmussen, B.B., et al., J. Clin. Invest. 110(11):1687-1693 (2002).
  • Ogawa, E., et al., J. Hum. Genet. 47(7):342-347 (2002).
  • Cook, G.A., et al., Am. J. Med. Sci. 318(1):43-48 (1999).
  • IJlst, L., et al., J. Clin. Invest. 102(3):527-531 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CPT1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 606-636 amino acids from the C-terminal region of human CPT1A.
NCBI Gene ID #:
1374
NCBI Official Name:
Carnitine O-palmitoyltransferase 1, liver isoform
NCBI Official Symbol:
CPT1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
88 kDa
Protein Accession #:
P50416
Protein GI Number:
56405343
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P50416
User NOte:
Optimal dilutions for each application to be determined by the researcher.