TOP2A Antibody
| Code | Size | Price |
|---|
| PSI-58-309-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a DNA topoisomerase, an enzyme that
controls and alters the topologic states of DNA during
transcription. This nuclear enzyme is involved in processes such as
chromosome condensation, chromatid separation, and the relief of
torsional stress that occurs during DNA transcription and
replication. It catalyzes the transient breaking and rejoining of
two strands of duplex DNA which allows the strands to pass through
one another, thus altering the topology of DNA. Two forms of this
enzyme exist as likely products of a gene duplication event. The
gene encoding this form, alpha, is localized to chromosome 17 and
the beta gene is localized to chromosome 3. The gene encoding this
enzyme functions as the target for several anticancer agents and a
variety of mutations in this gene have been associated with the
development of drug resistance. Reduced activity of this enzyme may
also play a role in ataxia-telangiectasia.
Background References:
- Vranic, S., et al. Hum. Pathol. 41(11):1617-1623(2010)
- Chen, H., et al. Am. J. Surg. Pathol. 34(9):1250-1257(2010)
- Ye, J., et al. Cell 142(2):230-242(2010)
- Rossi, E., et al. Histopathology 57(1):81-89(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TOP2A/Topo? antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1434-1461 amino acids from the C-terminal region of human TOP2A/Topo?.
NCBI Gene ID #:
7153
NCBI Official Name:
DNA topoisomerase 2-alpha
NCBI Official Symbol:
TOP2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
174 kDa
Protein Accession #:
P11388
Protein GI Number:
13959709
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P11388
User NOte:
Optimal dilutions for each application to be determined by the researcher.