ATF3 Antibody

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ProSci
Product Code: PSI-58-581
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-581-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).
2 / 3
Western blot analysis of ATF3 using rabbit polyclonal ATF3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.
3 / 3
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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis of ATF3 using rabbit polyclonal ATF3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.

Documents

Further Information

Additional Names:
Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Lee, S.H., et al. Oncogene 29(37):5182-5192(2010)
  • Park, H.J., et al. Biochem. Biophys. Res. Commun. 400(1):72-77(2010)
  • Wu, X., et al. Nature 465(7296):368-372(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Bovine
Immunogen:
This ATF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the C-terminal region of human ATF3.
NCBI Gene ID #:
467
NCBI Official Name:
Cyclic AMP-dependent transcription factor ATF-3
NCBI Official Symbol:
ATF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P18847
Protein GI Number:
1168543
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P18847
User NOte:
Optimal dilutions for each application to be determined by the researcher.