MSH2 Antibody

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ProSci
Product Code: PSI-55-920
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-920-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in SW480,U251 cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of MSH2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in SW480,U251 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of MSH2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq].
Background References:
  • Kim, M., et al. Cancer Sci. 101(11):2436-2442(2010)
  • Mangoni, M., et al. Int. J. Radiat. Oncol. Biol. Phys. (2010) In press :
  • Srivastava, K., et al. Cancer 116(13):3160-3169(2010)
  • van der Post, R.S., et al. J. Med. Genet. 47(7):464-470(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MSH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 637-665 amino acids from the Central region of human MSH2.
NCBI Gene ID #:
4436
NCBI Official Name:
DNA mismatch repair protein Msh2
NCBI Official Symbol:
MSH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
105 kDa
Protein Accession #:
P43246
Protein GI Number:
1171032
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P43246
User NOte:
Optimal dilutions for each application to be determined by the researcher.