CKAP4 Antibody

ProSci
Product Code: PSI-56-384
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-384-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
CKAP4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
2 / 2
Western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).

CKAP4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in 293,NCI-H460 cell line lysates (35ug/lane).

Further Information

Additional Names:
Cytoskeleton-associated protein 4, 63-kDa cytoskeleton-linking membrane protein, Climp-63, p63, CKAP4
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
CKAP4 is a substrate of DHHC2, a putative tumor suppressor. CK MNF116 and CKAP4 were useful in identifying squamous cell carcinomas with single cell infiltration. These results suggest a role for CKAP4 in tumor.
Background References:
  • Kanner, W.A., et al. J. Cutan. Pathol. 37(7):744-750(2010)
  • Tomkova, H., et al. EurDermatol 20(3):411-413(2010)
  • Lefkimmiatis, K., et al. Cancer Res. 69(22):8563-8571(2009)
  • Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CKAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 574-602 amino acids from the C-terminal region of human CKAP4.
NCBI Gene ID #:
10970
NCBI Official Name:
Cytoskeleton-associated protein 4
NCBI Official Symbol:
CKAP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q07065
Protein GI Number:
74735614
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q07065
User NOte:
Optimal dilutions for each application to be determined by the researcher.