MAGED2 Antibody

ProSci
Product Code: PSI-62-158
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-158-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Antibody is used in Western blot to detect MAGED2 in A549 lysate.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Antibody is used in Western blot to detect MAGED2 in A549 lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Melanoma-associated antigen D2, 11B6, Breast cancer-associated gene 1 protein, BCG-1, Hepatocellular carcinoma-associated protein JCL-1, MAGE-D2 antigen, MAGED2, BCG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
MAGED2 is a member of the MAGED gene family. In contrast to the MAGEA and MAGEB genes, which have their entire coding sequences located in the last exon, the MAGED genes have their coding sequences located throughout multiple exons. Also, the MAGEA and MAGEB genes are silent in normal tissues with the exception of testis and placenta, whereas the MAGED genes are expressed ubiquitously. The MAGED genes are clustered on chromosome Xp11. This gene is located in Xp11.2, a hot spot for X-linked mental retardation (XLMR).
Background References:
  • Lucas, S., et al., Cancer Res. 59(16):4100-4103 (1999).
  • Langnaese, K., et al., Cytogenet. Cell Genet. 94 (3-4), 233-240 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAGED2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from the C-terminal region of human MAGED2.
NCBI Gene ID #:
10916
NCBI Official Name:
Melanoma-associated antigen D2
NCBI Official Symbol:
MAGED2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
65 kDa
Protein Accession #:
Q9UNF1
Protein GI Number:
17380153
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
Q9UNF1
User NOte:
Optimal dilutions for each application to be determined by the researcher.