PDGFRL Antibody

ProSci
Product Code: PSI-63-123
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-123-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-PDGFRL Antibody in Hela cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human colon carcinoma reacted with PDGFRL antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PDGFRL using rabbit polyclonal PDGFRL-T343 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDGFRL gene.

Western blot analysis of anti-PDGFRL Antibody in Hela cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with PDGFRL antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PDGFRL using rabbit polyclonal PDGFRL-T343 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PDGFRL gene.

Further Information

Additional Names:
Platelet-derived growth factor receptor-like protein, PDGFR-like protein, PDGF receptor beta-like tumor suppressor, PDGFRL, PRLTS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PDGFRL bears significant sequence similarity to the ligand binding domain of platelet-derived growth factor receptor beta. Mutations in the PDGFRL gene, or deletion of a chromosomal segment containing this gene, are associated with sporadic hepatocellular carcinomas, colorectal cancers, and non-small cell lung cancers. This suggests PDGFRL may function as a tumor suppressor.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDGFRL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 328-357 amino acids from the C-terminal region of human PDGFRL.
NCBI Gene ID #:
5157
NCBI Official Name:
Platelet-derived growth factor receptor-like protein
NCBI Official Symbol:
PDGFRL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q15198
Protein GI Number:
74762141
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
Q15198
User NOte:
Optimal dilutions for each application to be determined by the researcher.