SPARCL1 Antibody

ProSci
Product Code: PSI-55-087
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-087-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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SPARCL1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
SPARCL1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
SPARC-like protein 1, High endothelial venule protein, Hevin, MAST 9, SPARCL1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background References:
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
  • Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
  • Kahler, A.K., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (7), 1089-1100 (2008) :
  • Weimer, J.M., et al. Glia 56(9):935-941(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SPARCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-236 amino acids from the Central region of human SPARCL1.
NCBI Gene ID #:
8404
NCBI Official Name:
SPARC-like protein 1
NCBI Official Symbol:
SPARCL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q14515
Protein GI Number:
259016170
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q14515
User NOte:
Optimal dilutions for each application to be determined by the researcher.