FOXP1 Antibody

Contact us
ProSci
Product Code: PSI-64-145
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-145-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Immunohistochemical analysis of paraffin-embedded H. thymus section using FOXP1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 4
Western blot analysis in A549,Daudi,Jurkat,MCF-7,NCI-H292 cell line lysates (35ug/lane).
3 / 4
4 / 4
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Immunohistochemical analysis of paraffin-embedded H. thymus section using FOXP1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in A549,Daudi,Jurkat,MCF-7,NCI-H292 cell line lysates (35ug/lane).
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Forkhead box protein P1, Mac-1-regulated forkhead, MFH, FOXP1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s).
Background References:
  • Johansson, A., et al. Obesity (Silver Spring) 18(4):803-808(2010)
  • Baro, C., et al. Histol. Histopathol. 24(11):1399-1404(2009)
  • Rayoo, M., et al. J. Clin. Pathol. 62(10):896-902(2009)
  • Courts, C., et al. J. Neuropathol. Exp. Neurol. 68(9):972-976(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This FOXP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 650-677 amino acids from the C-terminal region of human FOXP1.
NCBI Gene ID #:
27086
NCBI Official Name:
Forkhead box protein P1
NCBI Official Symbol:
FOXP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q9H334
Protein GI Number:
14548062
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q9H334
User NOte:
Optimal dilutions for each application to be determined by the researcher.