GPA33 Antibody

ProSci
Product Code: PSI-63-689
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-689-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35ug/lane)
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Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of GPA33 Antibody in K562, 293, MDA-MB231 cell line lysates (35ug/lane)
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Cell surface A33 antigen, Glycoprotein A33, GPA33
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
GPA33 is a cell surface antigen that is expressed in greater than 95% of human colon cancers. It may play a role in cell-cell recognition and signaling.
Background References:
  • Moritz,R.L., et.al.,Chromatogr798 (1-2), 91-101 (1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GPA33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human GPA33.
NCBI Gene ID #:
10223
NCBI Official Name:
Cell surface A33 antigen
NCBI Official Symbol:
GPA33
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q99795
Protein GI Number:
2842765
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q99795
User NOte:
Optimal dilutions for each application to be determined by the researcher.