ALKBH8 Antibody

ProSci
Product Code: PSI-62-476
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-476-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Alkylated DNA repair protein alkB homolog 8, 11411-, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8, ALKBH8, ABH8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis.
Background References:
  • Shimada,K., et.al., Cancer Res. 69 (7), 3157-3164 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ALKBH8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8.
NCBI Gene ID #:
91801
NCBI Official Name:
Alkylated DNA repair protein alkB homolog 8
NCBI Official Symbol:
ALKBH8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q96BT7
Protein GI Number:
189027650
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
Q96BT7
User NOte:
Optimal dilutions for each application to be determined by the researcher.