PYHIN1 Antibody

ProSci
Product Code: PSI-61-908
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-908-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
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PYHIN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
PYHIN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Pyrin and HIN domain-containing protein 1, Interferon-inducible protein X, PYHIN1, IFIX
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PYHIN1 belongs to the HIN200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-terminal ends. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis (Ding et al., 2006 [PubMed 16479015]).
Background References:
  • Yamaguchi, H., et al. Mol. Carcinog. 47(10):739-743(2008)
  • Ding, Y., et al. Mol. Cell. Biol. 26(5):1979-1996(2006)
  • Ding, Y., et al. Oncogene 23(26):4556-4566(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PYHIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human PYHIN1.
NCBI Gene ID #:
149628
NCBI Official Name:
Pyrin and HIN domain-containing protein 1
NCBI Official Symbol:
PYHIN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
Q6K0P9
Protein GI Number:
74748871
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q6K0P9
User NOte:
Optimal dilutions for each application to be determined by the researcher.