GSDMC Antibody

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ProSci
Product Code: PSI-55-406
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-406-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 3
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: MKN45 whole cell lysate Lane 3: SK-MEL-2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Overlay histogram showing U-2OS cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: MKN45 whole cell lysate Lane 3: SK-MEL-2 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Gasdermin-C, Melanoma-derived leucine zipper-containing extranuclear factor, GSDMC, MLZE
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100
Background References:
  • Birnbaum, S., et al. Nat. Genet. 41(4):473-477(2009)
  • Saeki, N., et al. Genes Chromosomes Cancer 48(3):261-271(2009)
  • Tamura, M., et al. Genomics 89(5):618-629(2007)
  • Watabe, K., et al. Jpn. J. Cancer Res. 92(2):140-151(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GSDMC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human GSDMC.
NCBI Gene ID #:
56169
NCBI Official Name:
Gasdermin-C
NCBI Official Symbol:
GSDMC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
Q9BYG8
Protein GI Number:
311033444
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
Q9BYG8
User NOte:
Optimal dilutions for each application to be determined by the researcher.