HDGF Antibody

ProSci
Product Code: PSI-62-269
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-269-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of anti-HDGF Pab in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HDGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Hepatoma-derived growth factor, HDGF, High mobility group protein 1-like 2, HMG-1L2, HDGF, HMG1L2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
HDGF is a member of the hepatoma-derived growth factor family. This protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation.
Background References:
  • Thakar,K., FEBS J. 275 (7), 1411-1426 (2008)
  • Chang,K.C.,Int. J. Cancer 121 (5), 1059-1065 (2007)
  • Yamamoto,S., Ann. Surg. Oncol. 14 (7), 2141-2149 (2007)
  • Uyama,H., Clin. Cancer Res. 12 (20 PT 1), 6043-6048 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HDGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-197 amino acids from the C-terminal region of human HDGF.
NCBI Gene ID #:
3068
NCBI Official Name:
Hepatoma-derived growth factor
NCBI Official Symbol:
HDGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
P51858
Protein GI Number:
1708157
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
P51858
User NOte:
Optimal dilutions for each application to be determined by the researcher.