EEF1A1 Antibody

ProSci
Product Code: PSI-62-352
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-352-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EEF1A1 Antibody in MCF-7 cell line lysates (35ug/lane)
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Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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EEF1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of EEF1A1 Antibody in MCF-7 cell line lysates (35ug/lane)
Flow cytometric analysis of NCI-H292 cells using EEF1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
EEF1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A1, EEF1A, EF1A, LENG7
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Background References:
  • Byun,H.O., Cancer Res. 69 (11), 4638-4647 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Hamster, Mouse, Rabbit, Rat, Chicken, Zebrafish
Immunogen:
This EEF1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the C-terminal region of human EEF1A1.
NCBI Gene ID #:
1915
NCBI Official Name:
Elongation factor 1-alpha 1
NCBI Official Symbol:
EEF1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P68104
Protein GI Number:
55584035
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer
Swissprot #:
P68104
User NOte:
Optimal dilutions for each application to be determined by the researcher.