API5 Antibody

ProSci
Product Code: PSI-60-149
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-149-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).

Western blot analysis in CEM cell line lysates (35ug/lane).

Further Information

Additional Names:
Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL, API5
Application Note:
For WB starting dilution is: 1:1000
Background:
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Background References:
  • Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :
  • Ren, K., et al. Pathol. Oncol. Res. 16(2):229-237(2010)
  • Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
  • Rigou, P., et al. EMBO J. 28(11):1576-1588(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This API5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-510 amino acids from the C-terminal region of human API5.
NCBI Gene ID #:
8539
NCBI Official Name:
Apoptosis inhibitor 5
NCBI Official Symbol:
API5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
Q9BZZ5
Protein GI Number:
353526346
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9BZZ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.