Cyclin B1 Antibody

ProSci
Product Code: PSI-63-023
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-023-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of (S126) in A375 cell line lysates (35ug/lane)

Western blot analysis of (S126) in A375 cell line lysates (35ug/lane)

Further Information

Additional Names:
G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Application Note:
For WB starting dilution is: 1:1000
Background:
Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form the maturation-promoting factor (MPF).
Background References:
  • Yoshida, T., et al., Ann. Oncol. 15(2):252-256 (2004). Chang, D.C., et al., J. Biol. Chem. 278(39):37865-37873 (2003). Sanchez, V., et al., J. Virol. 77(24):13214-13224 (2003). Li, J.Q., et al., Int. J. Oncol. 22(5):1101-1110 (2003). Porter, L.A., et al., Blood 101(5):1928-1933 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Cyclin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from human Cyclin B1.
NCBI Gene ID #:
891
NCBI Official Name:
G2/mitotic-specific cyclin-B1
NCBI Official Symbol:
CCNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P14635
Protein GI Number:
116176
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P14635
User NOte:
Optimal dilutions for each application to be determined by the researcher.